In situ genotyping individual DNA molecules by target-primed rolling-circle amplification of padlock probes
نویسندگان
چکیده
منابع مشابه
Signal amplification of padlock probes by rolling circle replication.
Circularizing oligonucleotide probes (padlock probes) have the potential to detect sets of gene sequences with high specificity and excellent selectivity for sequence variants, but sensitivity of detection has been limiting. By using a rolling circle replication (RCR) mechanism, circularized but not unreacted probes can yield a powerful signal amplification. We demonstrate here that in order fo...
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BACKGROUND In situ detection is traditionally performed with long labeled probes often followed by a signal amplification step to enhance the labeling. Whilst short probes have several advantages over long probes (e.g. higher resolution and specificity) they carry fewer labels per molecule and therefore require higher amplification for detection. Furthermore, short probes relying only on hybrid...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2004
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth723